Purification and properties of hyaluronidase from bull sperm.
نویسندگان
چکیده
منابع مشابه
Properties of acrosomal hyaluronidase from bull spermatozoa. Evidence for its similarity to testicular hyaluronidase.
Sperm acrosomes contain hyaluronidase, the amount varying, respectively, from ram, rabbit, bull, human, boar, rat, and stallion sperm to rooster sperm that do not possess this enzyme. Hyaluronidase was partially purified from bull sperm acrosomal extracts by DEAE-cellulose chromatography. Bull sperm acrosomal hyaluronidase has an optimum pH of 3.75 and is not effected by freezing and thawing or...
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A soluble pyridine nucleotidase was first described in bull semen by Leone and Bonaduce (1). During studies on the specificity of this enzyme, we observed that passage of dilute semen through a column composed of calcium phosphate gel and diethylaminoethyl cellulose anion exchanger (Whatman)] resulted in a preparation of high specific activity. In this single purification step the specific acti...
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Bull seminal-plasma hyaluronidase was purified 180-fold by chromatography on concanvalin A-Sepharose, heparin Sepharose, Sephadex G-200 and Sephacryl S-200. With hyaluronic acid as the substrate, the specific activity and turnover number of purified hyaluronidase were 3.63 mumol/min per mg (104000 National Formulary units/mg of protein) and 214 min-1 (mol of product formed/mol of enzyme per min...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41982-0